The incorporation of radiolabelled fatty acids into the major phospholipids of M. hyorhinis.
Radioactivity (% of total)
% of total
M. hyorhinis cells were grown in the presence of either [3H]-palmitate or [3H]-oleate. Lipids were extracted by the method of Bligh and Dyer  and separated by TLC on silica gel plates (Kiesel-gel 60 HR, Merck, Darmstadt, Germany) developed at room temperature by a two-dimensional system described above. The lipid spots were scraped off the plates and analyzed for radioactivity. To determine phosphorus in the phospholipid spots, the method of Zhou and Arthur  was used. The results are the average of three independent experiments using different batches of cells. SPM: sphingomyelin; PC: phosphatidylcholine; PG: phosphatidylglycerol; CL: cardiolipin.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.