The Impairment of Macrophage-to-Feces Reverse Cholesterol Transport during Inflammation Does Not Depend on Serum Amyloid A
Figure 5
Intraperitoneal injection of J774 cells induces an acute phase response in C57BL/6 mice. (a) WT mice were injected subcutaneously with PBS or 0.8 μg/g LPS (−4 hr) followed 4 hr later by an intraperitoneal injection of 3H-cholesterol-labeled J774 macrophages (0 hr); per treatment). Aliquots of plasma (0.25 μL) collected from individual mice at the indicated times were subjected to SDS-PAGE, followed by immunoblotting for SAA. (b) In two parallel studies, C57BL/6 mice were injected subcutaneously with PBS or 0.8 μg/g LPS, and 4 hr later, the PBS-injected mice were injected i.p. with cholesterol-loaded J774 macrophages or C57BL/6 BMM, as indicated ( per treatment). Aliquots of plasma (0.25 μL) collected from individual mice 28 hr after PBS/LPS injection (i.e., 24 hr after macrophage injection) were subjected to SDS-PAGE, followed by immunoblotting for SAA. (c) Relative plasma SAA concentrations 28 hr after PBS/LPS injection (i.e., 24 hr after macrophage injection) as determined by densitometry (). Values are the mean ± SEM; ***; *.
