5-Lipoxygenase-Activating Protein as a Modulator of Olanzapine-Induced Lipid Accumulation in Adipocyte
Figure 3
Effect of olanzapine treatment on 5-LOX and FLAP mRNA contents. 3T3-L1 cells were differentiated into adipocytes in the presence and absence of 50 μM olanzapine. Samples (3 culture dishes per treatment and time point) were collected 24 hours and 5 days after the initiation of the differentiation protocol. The content of 5-LOX and FLAP mRNAs was quantified with a real-time PCR. Data (mean ± SEM) are expressed as units (coefficient of variation normalized to the corresponding cyclophilin internal control); * versus the corresponding vehicle-treated control.
