Incubation of MDCO-216 (ApoA-IMilano/POPC) with Human Serum Potentiates ABCA1-Mediated Cholesterol Efflux Capacity, Generates New Prebeta-1 HDL, and Causes an Increase in HDL Size

Figure 3

Changes in the ¹NMR lipid methyl signal envelope brought about by incubation of MDCO-216 with serum at 47°C. The signals shown are from MDCO-216 (dark blue) and the serum specimen (red) before they were mixed together. The digital sum of these 2 signals (black) represents the mixture at “0 min” before physical mixing occurs, and the blue and green curves are the signals observed after 2.8 and 10.8 minutes of incubation, respectively. Also the positions in the serum spectrum of the methyl signals from the subclasses of VLDL, LDL, and HDL, with larger subclasses giving rise to signals further to the left [8], are shown at the top.