Effects of Myeloperoxidase-Induced Oxidation on Antiatherogenic Functions of High-Density Lipoprotein
Effects of HDL treatment with and without MPO on THP-1 cell migration. The effects of HDL on THP-1 cell migration were determined using PET membranes (8 μm pore size) (a). HUVECs were cultured in medium containing (black columns) or lacking (white columns) LPS with MPO-treated (+) and MPO-untreated (−) HDL or without HDL (PBS) at 37°C for 16 hrs. The media used for HUVEC culture were then supplied to a THP-1 cell migration assay. The THP-1 cell migration rate in the absence of LPS and HDL (PBS) was defined as 1. The results are means ± SD of three independent experiments. , †. ns: nonsignificant. MCP-1 concentrations in those media used for THP-1 cell migration assay were measured in triplicate by ELISA kit (b). The results are means ± SD of one experiment. †. ns: nonsignificant.
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