Research Article

Cholesterol Efflux Capacity of Apolipoprotein A-I Varies with the Extent of Differentiation and Foam Cell Formation of THP-1 Cells

Figure 1

The morphological changes in THP-1 cells. THP-1 cells were treated with 100 ng/mL PMA for different duration (1 to 5 days). After treatment, (a) THP-1 cells were fixed with 3.7% formaldehyde and stained with hematoxylin (scale bars, 20 μm). (b) THP-1 cells were incubated with FITC-conjugated anti-CD11b antibody, and CD11b expression was analyzed using flow cytometry. The percentage of CD11b positive cells which exceeded the maximum fluorescent intensity of the isotype control in each day was indicated. The value at day 1 was defined as 1. The values were indicated by mean + SD ( = 5, < 0.01 versus day 1, < 0.05 versus day 1.5).
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