Cholesterol Efflux Capacity of Apolipoprotein A-I Varies with the Extent of Differentiation and Foam Cell Formation of THP-1 Cells

Figure 7

The effect of differentiation and foam cell formation on cholesterol efflux capacity of apoA-I and N-Hcy apoA-I. (a) After THP-1 cells were differentiated with PMA for different periods (1 to 5 days) and were loaded with ³H-cholesterol (1 μCi/mL), acLDL (50 μg/mL), and T0901317 (1 μmol/L) for 1 day, cholesterol efflux capacity was assessed by using apoA-I (10 μg/mL) or N-Hcy apoA-I (10 μg/mL). The values were indicated by mean + SD (, < 0.05). (b) After PMA treatment for 2 days and loading with ³H-cholesterol (1 μCi/mL), acLDL (50 μg/mL), and T0901317 (1 μmol/L) for different periods (1 to 5 days), cholesterol efflux capacity was determined by using apoA-I (10 μg/mL) or N-Hcy apoA-I (10 μg/mL). The values were indicated by mean + SD ( = 6, < 0.01).

(a)

(b)