Elucidation of the Role of Lectin-Like oxLDL Receptor-1 in the Metabolic Responses of Macrophages to Human oxLDL

Table 9

Metabolic effects of mouse LOX-1/OLR1 antibody 10 min exposure followed by 6 min exposure to Kalen high oxLDL on macrophages. Mean peak MAMP height changes and standard errors for each experimental condition during antibody exposure, oxLDL exposure, and recovery are shown. All values are calculated based on peak height versus 10 min average basal value prior to exposure. Terms in bold are representative of , indicating statistical significance.

Mouse LOX-1/OLR1 antibody and oxLDL exposure
		Glucose (%)	Lactate (%)	Oxygen (%)	Acidification (%)
1 μg/mL
	Antibody exposure	20.1 ± 17.8	3.15 ± 0.6	43.2 ± 39.9	7.9 ± 7.7
		= 0.9699	p = 0.0046	= 0.3284	= 0.4077
	oxLDL exposure	32.9 ± 34.3	5.1 ± 2.1	20.4 ± 13.3	5.27 ± 6.5
		= 0.446	= 0.0606	= 0.1868	= 0.5293
	Recovery	27.3 ± 44.0	6.61 ± 1.9	92.7 ± 56.1	29.7 ± 34.5
		= 0.5972	p = 0.0192	= 0.1594	= 0.4217
10 μg/mL
	Antibody exposure	1.92 ± 11.5	3.55 ± 0.91	17.34 ± 9.8	1.72 ± 0.97
		= 0.9584	p = 0.0109	= 0.1785	= 0.1754
	oxLDL exposure	14.8 ± 27.1	6.4 ± 0.99	10.8 ± 2.6	11.2 ± 4.2
		= 0.7436	p = 0.0007	= 0.1529	= 0.0759
	Recovery	12.3 ± 38.1	9.9 ± 1.1	0.2 ± 1.4	9.64 ± 6.9
		= 0.8174	p < 0.0001	= 0.9758	= 0.2568
50 μg/mL
	Antibody exposure	15.5 ± 20.8	5.7 ± 9.5	124.21 ± 124.2	4.7 ± 1.9
		= 0.781	= 0.5755	= 0.391	= 0.0995
	oxLDL exposure	14.0 ± 15.9	11.8 ± 8.9	141.9 ± 173.6	20.6 ± 10.9
		= 0.7947	= 0.2425	= 0.4737	= 0.1557
	Recovery	138.7 ± 104.2	1.1 ± 5.6	135.8 ± 166.9	40.2 ± 6.2
		= 0.2679	= 0.8496	= 0.4754	p = 0.0076
100 μg/mL
	Antibody exposure	1.85 ± 6.7	0.67 ± 0.25	34.4 ± 10.2	1.05 ± 1.51
		= 0.7939	= 0.2218	p = 0.0203	= 0.5369
	oxLDL exposure	8.56 ± 1.8	4.34 ± 2.4	30.4 ± 12.1	34.6 ± 30.1
		p = 0.0057	= 0.1348	= 0.0544	= 0.3337
	Recovery	18.5 ± 1.9	2.06 ± 1.9	10.3 ± 8.1	57.2 ± 48.6
		p = 0.0002	= 0.3377	= 0.2633	= 0.324