Review Article

Realm of Thermoalkaline Lipases in Bioprocess Commodities

Table 4

The inoculums size, medium composition, temperature, pH, production duration time, and activity of TA lipases.

Strain/originIS (%)Medium composition (%, w/v or v/v)Temp (°C), agitation (rpm)pHOther minerals (%, w/v or v/v)Duration (h)Activity (U/mL or other units)References

B. stearothermophilus1Xylose, 1; peptone, 1; olive oil, 155, 20011MgSO4; Tw-80481800–2500[26]
Geobacillus sp.DA24Galactose, 1; (NH4)3PO4,, 0.560, 12010-481000[54]
Bacillus sp.ID-1-Olive oil, 1.565, ---12520 (IU)[31]
C. thermotolerans5Maltose, 1; starch, 160, 120--72-[18]
B. methylotrophicus10NB; tributyrin, 140, -7Ca2+; Tw-80, 172360 (IU/mL)[50]
S. aureus ALA1-Xylose, 1; YE, 1; olive oil, 130, -8-30128[49, 51]
S. pasteuri2Coconut oil mill waste, 235, 1207-4820[8]
B. subtilis BSK-L1YE, 0.5; BE, 0.5; peptone, 137, 2007NaCl, 0.2; olive oil, 124120 (U/g)[22]
Microbacterium sp.-Tributyrin, 1; NaNO3, 0.0085; Fe(CH3COO)3NH4, 0.00537, -8.5MgSO4, 0.02; KH2PO4, 0.68; Na2HPO4·2H2O, 0.7872355 (U) or
3.2 (U/mg)
[23]
Aeribacillus sp. SSL-YE, 1; olive oil, 170, -8-7 d1.339 (µM/min)[59]
Bacillus sp. LBN 2aGroundnut oil, 1; peptone, 150, 2009NaCl, 74820 (U/g)[9]
B. sonorensis 4R5Tw-80, 1; glucose, 1; (NH4)SO4, 0.280, -9K2HPO4, MgSO4, NaCl, CaCO3, CaSO4, FeSO4, MnCl2, ZnCl2 (2 to 0.001)96177 (U/mg)[4]
B. licheniformis Ht7-Olive oil, 130, ---2415.9 (U/g)[32]
B. coagulans BTS-3-Mustard oil, 1; peptone : YE, 1 : 155, -8.5-481.16[63]
B. thermoleovorans1NB, 0.325; olive oil, 2.555, 1506.5CaCl2, 0.1%; gum Arabic, 1446000[64]
B. pumilus HF544325-Casein peptone, 1.7; YE, 0.537, 2007.4Glucose, 0.257214[60]
S. arlettae JPBW-110Soybean oil, 535, 1008-3-[55]
Pseudomonas sp2
b
Glucose, 1; NH4Cl, 0.5; ECO, 2; (NH4)2HPO4, 0.337, 2507K2HPO4, 0.3; KH2PO4, 0.1; MgSO4·7H2O, 0.012424 (U/mg)[21]
O. intermedium-YE, 0.5; olive oil, 1; NH4Cl, 0.160, 18010MgSO4·7H2O, FeSO4·7H2O, CaCl2·7H2O, K2HPO4 (1 to 0.1)72-[65]
T. thermophilus-Tributyrin-olive oil emulsion, 150, -9.5-7.3–10 (kU/mg)[20, 29]
T.atroviride 6761
c
Tw-80, 0.5; olive oil, 1; YE, 1528, 1056(NH4)2SO4, 0.25; MgSO4, 0.24 d175.20[33]
Curvularia sp. DHEdWheat bran, 130, -7Olive oil, 2; KCl, 0.05; MC, 707 d83.4 (U/g)[57]

Note. IS, inoculums size; NaCl, sodium chloride; K2HPO4, potassium hydrogen phosphate; KCl, potassium chloride; Na2S·9H2O, sodium sulphide; NH4Cl, ammonium chloride; MgCl2·6H2O, magnesium chloride hexahydrate; CaCl2·2H2O, calcium chloride dehydrate; NaHCO3, sodium bicarbonate; Na2CO3, sodium carbonate; C3H7NO2S, cysteine; (NH4)3PO4, ammonium phosphate; YE, yeast extract; BE, beef extract; a, 104 cells/mL; ECO, emulsified coconut oil; b, 14 h old culture (1.3 × 109 cell/ml); MC, moisture content; SSF, solid-state fermentation; c (106 spores/ml); d (51.27 × 107 spore/mL).