Journal of Lipids / 2018 / Article / Fig 2

Research Article

Mitochondrial-Targeted Antioxidant MitoQ Prevents E. coli Lipopolysaccharide-Induced Accumulation of Triacylglycerol and Lipid Droplets Biogenesis in Epithelial Cells

Figure 2

Incubation of FUBEC with LPS changes the distribution of [3H]-C18:1 between lipid classes. (a, b) Effect of LPS on the metabolism of different lipid classes. PL: phospholipids, TAG: triacylglycerols, DAG: diacylglycerols, CE: cholesterol esters. ((c), (d)) Time-course of the effect of LPS on [3H]-TAG level and on [3H]-C18:1 oxidation. Cells were preloaded with [3H]-С18:1 for 1 h and then rinsed and incubated with LPS (25 μg/ml, 21 h). At the end of the incubation, cells were harvested and total lipid extraction and TLC were performed. n=6 independent experiments. p< 0.01; p<0.001 versus control.

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