Research Article
Mitochondrial-Targeted Antioxidant MitoQ Prevents E. coli Lipopolysaccharide-Induced Accumulation of Triacylglycerol and Lipid Droplets Biogenesis in Epithelial Cells
Figure 6
MitoQ decreases basal and LPS-stimulated ROS production (a) and prevented LPS-induced TAG accumulation (b), decline of FAO (c), and LD biogenesis (d-h). (a) Cells were pretreated with MitoQ for 2 h and then exposed to 25 μg/ml LPS for 1 h. The fluorescent dye, DCF-DA, was added to the incubation medium at a final concentration of 10 μM, 20 min before the end of incubation with LPS, n = 6. ((b)-(h)) Cells were pretreated for 2 h with MitoQ (25 nM) and then incubated for 21 h with 25 μg/ml LPS. ((b), (c)) Cells were preloaded with [3H]-С18:1 during 1 h, n=6 independent experiments; ((d)-(h)) Cells were stained with Nile Red, n=4 independent experiments. p< 0.05; p< 0.01.
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |
(h) |