Scavenger Receptor Class B Member 1 Independent Uptake of Transthyretin by Cultured Hepatocytes Is Regulated by High Density Lipoprotein

Figure 4

Scavenger receptor Class B Type 1 (SR-B1) levels affect uptake of Alexa-TTR-T4 by HepG2 cells. (a) Knockdown of SR-B1 levels (to 29±8% of mock control levels) prior to uptake experiments resulted in increased (~133%) uptake of Alexa-TTR-T4 at 48 hours compared to control. (b) Comparison of Alexa-TTR (square) uptake in mock control (blue) versus SR-B1 knockdown (orange) cells and of AlexaTTR-T4 (circle) uptake in mock control versus SR-B1 knockdown cells (all n=4, p<0.05).

(a)

(b)