Phylogeography of the Pacific Blueline Surgeonfish, Acanthurus nigroris, Reveals High Genetic Connectivity and a Cryptic Endemic Species in the Hawaiian Archipelago

Table 2

Genetic structuring (Analysis of Molecular Variance, AMOVA) of Acanthurus nigroris sampled at sites throughout the Pacific based on 797 bp of mtDNA cyt b sequence data (𝑁=539). All Hawaiian populations (including Johnston Atoll) and the remaining Pacific populations were divided into two separate groups to assess the relationship between these regions. Φ_CT: region variance component relative to total variance; Φ_SC: between population within region variance component divided by the sum of itself and within population variance; Φ_ST: sum of the variance due to region and population within region divided by the total variance.

Source	d.f.	SS	Variance; components	%; variation	ΦCT; ΦSC	𝑃 value	ΦST	P value
Among regions; (Hawaii versus Pacific)	1	2543.54	15.86	95.74	𝟎.𝟗𝟔^a	<0.001	𝟎.𝟗𝟔	<0.001
Among populations; (within regions)	15	15.11	0.0097	0.06	𝟎.𝟎𝟏𝟒	0.035
Within populations	522	363.35	0.70	4.2

aNumbers in bold are significant, 𝑃<0.05.