Screening of Genes Specifically Expressed in Males of Fenneropenaeus chinensis and Their Potential as Sex Markers

Table 1

List of primers with their information used for RT-PCR.


Gene symbol	Gene common name	Primers -	(°C)	Product size (bp)

Contig10	Unknown	F-AAGTGGTGCTAGTCGTTGC	54	336
		R-GGAGGGTCCTGCTTCTGT
Contig14	Unknown	F-TGTGGACCTGCCTTCTT	55	342
		R-CGTATTTAGTGGCTGAGTG
Contig16	Unknown	F-GTGCTGGCGTCAGAGGGTAT	60	539
		R-TTGGTTAGATCGGTGAGGAGTC
Contig19	Unknown	F-GTGAAACTGTGAGACTTATCCCTGT	60	152
		R-GCAACTACGAGATGAAGAATCCAAT
Contig26	Unknown	F-AGAAGCGAGAATCATAAGAGG	53	213
		R-TACGGCAGTTGTCCAGAA
Contig31	Unknown	F-CGTCCAGGTTTCATCGC	55	408
		R-CACGGAGGCATCCATCT
Contig32	Unknown	F-GAGGTTCTGATAAAGTCGTGGGT	60	119
		R-GTATGCGTTGATTCTCGTAGCG
Contig39	Unknown	F-ATGCTCCTCTTTGCTCTTCCGT	62	327
		R-TGTTATCTGTGTCGCCTTTGCC
Contig47	Unknown	F-GAACCTGCTGAGGAACTTGAATGT	62	158
		R-TCCCCAACCAGTTCAGCCTAC
Contig48	Unknown	F-GAGGTTCTGATAAAGTCGTGG	54	138
		R-GTCTGTTGCTGGCGATG
CHH	Crustacean hyperglycemic hormone	F-GTTTTGTCCTCAAGCCTCTCTC	57	289
		R-GCGGAGTGGGACTACTTACC
18S-rRNA	18S-rRNA	F-TCTGCCCTATCAACTTTCGATGGTA	64	257
		R-AATTTGCGCGCCTGCTGCCTTCCTT

F: forward; R: reverse; : annealing temperature.