Table of Contents
Journal of Mycology
Volume 2014, Article ID 324349, 5 pages
Research Article

Diversity of Biscogniauxia mediterranea within Single Stromata on Cork Oak

1Instituto Nacional de Investigação Agrária e Veterinária, I.P. Unidade Estratégica de Sistemas Agrários e Florestais e Sanidade Vegetal, Quinta do Marquês, 2780-159 Oeiras, Portugal
2Centro de Engenharia dos Biossistemas, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal

Received 18 July 2014; Accepted 3 October 2014; Published 14 October 2014

Academic Editor: Massimo Cogliati

Copyright © 2014 Joana Henriques et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Charcoal canker, caused by the fungus Biscogniauxia mediterranea, is one of the most frequent diseases of cork oak in Portugal. The pathogen has been considered a secondary invader that attacks only stressed hosts; however, in recent years, an increasing number of young trees exhibiting the disease symptoms have been recorded. A collection of monoascosporic cultures isolated from single stromata of B. mediterranea in cork oak from different locations was analyzed by means of microsatellite—Primed Polymerase Chain Reaction—using three microsatellite primers, in order to detect the genetic variation of the population thus discussing its plasticity and ability to adapt to different conditions. The results showed a high level of genetic variability among isolates obtained from the same stroma, being impossible to distinguish isolates from individual stromata neither from different geographical location.