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Journal of Nucleic Acids
Volume 2010, Article ID 121259, 7 pages
http://dx.doi.org/10.4061/2010/121259
Research Article

Cation Involvement in Telomestatin Binding to G-Quadruplex DNA

1Mass Spectrometry Laboratory, Department of Chemistry, University of Liège, 4000 Liège, Belgium
2GIGA-Systems Biology and Chemical Biology, University of Liège, 4000 Liège, Belgium
3Biomedicinal Information Research Center, Biological Systems Control Team, National Institute of Advanced Industrial Science and Technology, Tokyo 135-0064, Japan

Received 1 February 2010; Accepted 13 April 2010

Academic Editor: Daniela Montesarchio

Copyright © 2010 Frédéric Rosu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The binding mode of telomestatin to G-quadruplex DNA has been investigated using electrospray mass spectrometry, by detecting the intact complexes formed in ammonium acetate. The mass measurements show the incorporation of one extra ammonium ion in the telomestatin complexes. Experiments on telomestatin alone also show that the telomestatin alone is able to coordinate cations in a similar way as a crown ether. Finally, density functional theory calculations suggest that in the G-quadruplex-telomestatin complex, potassium or ammonium cations are located between the telomestatin and a G-quartet. This study underlines that monovalent cation coordination capabilities should be integrated in the rational design of G-quadruplex binding ligands.