Divalent Metal- and High Mobility Group N Protein-Dependent Nucleosome Stability and Conformation

Figure 3

S2 formation is divalent metal dependent and requires HMGN elements outside of the NBD. EMSA gel labels indicate HMGN identity (N1t, HMGN1t; N1, HMGN1; N2, HMGN2; a, 12-array alone; n, NCP alone), and numbers designate HMGN : nucleosome molar stoichiometry. (a) HMGN binding to 12-array at near physiological ionic strength with 1 mM Ca²⁺. (b) Binding of HMGN proteins and NBD peptides to NCP-601 at near physiological ionic strength with 1 mM Mg²⁺. (c) Binding of HMGN NBD peptides to 12-array at low ionic strength (top), near physiological ionic strength (middle), and near physiological ionic strength with 1 mM Mg²⁺ (bottom).

(a)

(b)

(c)