Research Article
Effect of Ionic Strength on Porphyrin Drugs Interaction with Quadruplex DNA Formed by the Promoter Region of C-myc and Bcl2 Oncogenes
(a) Effect of ionic strength on the percentage of fluorescence hypochromicity while 27Bcl2 interacts with porphyrin drugs. |
| S. No. | Ionic strength of KBPES buffer (pH7.0) | Stoichiometry DNA : drug | Percentage of fluorescence hypochromicity |
| 1. | TMPyP4 with 27BCL2 | 1.a | 30 mM | | 34.2% | 1.b | 100 mM | | 28.5% | 1.c | 200 mM | | 20.3% | 1.d | 600 mM | | 6.5% |
| 2. | TMPyP3 with 27BCL2 | 2.a | 30 mM | | 59.8% | | 51.5% | 2.b | 100 mM | | 52.5% | | 49.5% | 2.c | 200 mM | | 33.6% | | 16.6% | 2.d | 600 mM | | 8.6% | | 4.9% |
| 3. | TMPyP2 with 27BCL2 | 3.a | 30 mM | | 37.4% | | 40.2% | 3.b | 100 mM | | 11.9% | | 12.3% | 3.c | 200 mM | | 6.5% | | 4.4% | 3.d | 600 mM | | 0.7% | | 0.3% |
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(b) Effect of ionic strength on the percentage of fluorescence hypochromicity while C-myc interacts with porphyrin drugs. |
| S. No. | Ionic strength of KBPES buffer (pH7.0) | Stoichiometry DNA : drug | Percentage of fluorescence hypochromicity |
| 1. | TMPyP4 with C-myc | 1.a | 30 mM | | 39.2% | 1.b | 100 mM | | 32.6% | 1.c | 200 mM | | 29.3% | 1.d | 600 mM | | 5.5% |
| 2. | TMPyP3 with C-myc | 2.a | 30 mM | | 52.8% | | 49.5% | 2.b | 100 mM | | 47.5% | | 42.2% | 2.c | 200 mM | | 29.6% | | 12.6% | 2.d | 600 mM | | 6.6% | | 3.9% |
| 3. | TMPyP2 with C-myc | 3.a | 30 mM | | 42.4% | | 37.2% | 3.b | 100 mM | | 12.6% | | 7.2% | 3.c | 200 mM | | 7.8% | | 5.4% | 3.d | 600 mM | | 0.6% | | 0.2% |
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