Journal of Nucleic Acids

Research Article

Effect of Ionic Strength on Porphyrin Drugs Interaction with Quadruplex DNA Formed by the Promoter Region of C-myc and Bcl2 Oncogenes

(a) Effect of ionic strength on the percentage of fluorescence hypochromicity while 27Bcl2 interacts with porphyrin drugs.


S. No.	Ionic strength of KBPES buffer (pH7.0)	Stoichiometry DNA : drug	Percentage of fluorescence hypochromicity

1.	TMPyP4 with 27BCL2
1.a	30 mM		34.2%
1.b	100 mM		28.5%
1.c	200 mM		20.3%
1.d	600 mM		6.5%

2.	TMPyP3 with 27BCL2
2.a	30 mM		59.8%
			51.5%
2.b	100 mM		52.5%
			49.5%
2.c	200 mM		33.6%
			16.6%
2.d	600 mM		8.6%
			4.9%

3.	TMPyP2 with 27BCL2
3.a	30 mM		37.4%
			40.2%
3.b	100 mM		11.9%
			12.3%
3.c	200 mM		6.5%
			4.4%
3.d	600 mM		0.7%
			0.3%

(b) Effect of ionic strength on the percentage of fluorescence hypochromicity while C-myc interacts with porphyrin drugs.


S. No.	Ionic strength of KBPES buffer (pH7.0)	Stoichiometry DNA : drug	Percentage of fluorescence hypochromicity

1.	TMPyP4 with C-myc
1.a	30 mM		39.2%
1.b	100 mM		32.6%
1.c	200 mM		29.3%
1.d	600 mM		5.5%

2.	TMPyP3 with C-myc
2.a	30 mM		52.8%
			49.5%
2.b	100 mM		47.5%
			42.2%
2.c	200 mM		29.6%
			12.6%
2.d	600 mM		6.6%
			3.9%

3.	TMPyP2 with C-myc
3.a	30 mM		42.4%
			37.2%
3.b	100 mM		12.6%
			7.2%
3.c	200 mM		7.8%
			5.4%
3.d	600 mM		0.6%
			0.2%