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Journal of Nucleic Acids
Volume 2010, Article ID 147528, 6 pages
Research Article

Expression Levels of Some Antioxidant and Epidermal Growth Factor Receptor Genes in Patients with Early-Stage Non-Small Cell Lung Cancer

1Department of Experimental and Applied Medicine, Section of Occupational Health and Industrial Hygiene, University of Brescia, Piazzale Spedali Civili, 1, 25123 Brescia, Italy
2ISPESL Research Center at the University of Parma, via Gramsci 14, 43100 Parma, Italy
3Laboratory of Industrial Toxicology, Department of Clinical Medicine Nephrology and Health Sciences, University of Parma, via Gramsci 14, 43100 Parma, Italy
4Thoracic Surgery Unit, University of Parma, via Gramsci 14, 43100 Parma, Italy
5Division of Medical Oncology, University of Parma, via Gramsci 14, 43100 Parma, Italy

Received 24 November 2009; Revised 21 January 2010; Accepted 27 January 2010

Academic Editor: Hiroshi Sugiyama

Copyright © 2010 Giuseppe De Palma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study was aimed at: (i) investigating the expression profiles of some antioxidant and epidermal growth factor receptor genes in cancerous and unaffected tissues of patients undergoing lung resection for non-small cell lung cancer (NSCLC) (cross-sectional phase), (ii) evaluating if gene expression levels at the time of surgery may be associated to patients' survival (prospective phase). Antioxidant genes included heme oxygenase 1 (HO-1), superoxide dismutase-1 (SOD-1), and -2 (SOD-2), whereas epidermal growth factor receptor genes consisted of epidermal growth factor receptor (EGFR) and v-erb-b2 erythroblastic leukaemia viral oncogene homolog 2 (HER-2). Twenty-eight couples of lung biopsies were obtained and gene transcripts were quantified by Real Time RT-PCR. The average follow-up of patients lasted about 60 months. In the cancerous tissues, antioxidant genes were significantly hypo-expressed than in unaffected tissues. The HER-2 transcript levels prevailed in adenocarcinomas, whereas EGFR in squamocellular carcinomas. Patients overexpressing HER-2 in the cancerous tissues showed significantly lower 5-year survival than the others.