Parameters of Reserpine Analogs That Induce MSH2/MSH6-Dependent Cytotoxic Response
Figure 2
Combination treatment of cisplatin and rescinnamine drastically reduces cell viability within 24 hours (a) and completely eliminates all cells at 48 and 72 hours (b and c, resp.) when cells are treated with cisplatin (10 M) and increasing concentrations of rescinnamine (0–25 M). Cell viability is accessed via MTS assays; MSH2-deficient cells (■) and MSH2-proficient (▴) cell response is shown. Mean values and standard deviations of three independent experiments are shown. (d) Immunoblotting demonstrates caspase-3 cleavage in Hec59 (MSH2-deficient; lanes 1 and 2) and Hec59(2) (MSH2-proficient; lanes 3 and 4) cells upon treatment with 24 hours of cisplatin (10 M) and rescinnamine (60 M). The 17 and 19 kDa cleavage products of caspase-3 are depicted. Staurosporine (STS) was used as a positive control; actin was used as a loading control.
