Journal of Nucleic Acids / 2010 / Article / Fig 7

Review Article

DNA-Destabilizing Agents as an Alternative Approach for Targeting DNA: Mechanisms of Action and Cellular Consequences

Figure 7

Nuclease S1 mapping of locally opened DNA structure. Increasing concentrations of the benzo-b-acronycine derivatives presented in Figure 5 were incubated with a radio-labelled 117-bp DNA fragment prior to subjection to nuclease S1 mapping of the induced locally single-stranded DNA portions generated upon DNA alkylation. DNA samples were separated on a 10% native polyacrylamide gel. Concentrations are expressed in 𝜇 M. The detailed experimental protocol is described in [91].

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