DNA-Destabilizing Agents as an Alternative Approach for Targeting DNA: Mechanisms of Action and Cellular Consequences
Nuclease S1 mapping of locally opened DNA structure. Increasing concentrations of the benzo-b-acronycine derivatives presented in Figure 5 were incubated with a radio-labelled 117-bp DNA fragment prior to subjection to nuclease S1 mapping of the induced locally single-stranded DNA portions generated upon DNA alkylation. DNA samples were separated on a 10% native polyacrylamide gel. Concentrations are expressed in M. The detailed experimental protocol is described in .
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.