Targeting the OB-Folds of Replication Protein A with Small Molecules
Figure 5
Irreversible inactivation of full-length heterotrimeric RPA by MCI13E. Incubation of RPA with MCI13E, dialysis, and recovery were performed as described in the legend to Figure 3. DNA binding activity of the resulting protein was assessed by EMSA using a 5′-[32P]-ss 34-base DNA as described in “Section 2.” Lanes 1–4 predialysis, lanes 5 and 6 postdialysis.
