Figure 1: Generation of stable cell lines expressing GFP-tagged proteins. (a) Stepwise protocol for stable expression of GFP-fusion proteins in cells. (b) 293T cells were transfected with GFP-Rad1, Rad1-GFP, or mock transfected, after which cells were lysed and immunoprecipitations were carried out using anti-GFP antibody. Western blot analysis of the immunoprecipitates using the indicated antibodies. (c) Protein expression analysis of different stable clones. Western blot analysis of whole cell extracts using the indicated antibodies.