Repair of DNA Alkylation Damage by the Escherichia coli Adaptive Response Protein AlkB as Studied by ESI-TOF Mass Spectrometry

Figure 3

ESI-TOF analysis of 16mer reactants and products from the in vitro incubation reactions of alkylated lesions and AlkB protein. Data represent the −4 charge envelopes; multiple ion mass peaks associated with each envelope reflect mostly the number of ¹³C atoms in each −4 charged packet. The monoisotopic peak (all ¹²C, ¹⁴N, etc.) value is labeled above each peak envelope. (a) 1MeG; (b) 1MeG + AlkB; (c) 𝑂⁶MeG (TXG); (d) 𝑂⁶MeG (TXG) + AlkB; (e) 𝑂⁶MeG (GXT); (f) 𝑂⁶MeG (GXT) + AlkB; (g) 1MeA; (h) 1MeA + AlkB; (i) eA; (j) eA + AlkB.