Repair of DNA Alkylation Damage by the Escherichia coli Adaptive Response Protein AlkB as Studied by ESI-TOF Mass Spectrometry

Figure 5

ESI-TOF analysis of 16 mer reactants and products from the in vitro incubation reactions of alkylated lesions and AlkB protein. Data represent the −4 charge envelopes; multiple ion mass peaks associated with each envelope reflect mostly the number of ¹³C atoms in each −4 charged packet. The monoisotopic peak (all ¹²C, ¹⁴N, etc.) value is labeled above each peak envelope. (a) 3MeC; (b) 3MeC + AlkB; (c) 3EtC; (d) 3EtC + AlkB; (e) 5MeC; (f) 5MeC + AlkB; (g) eC; (h) eC + AlkB; (i) 3MeT; (j) 3MeT + AlkB.