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Journal of Nucleic Acids
Volume 2010 (2010), Article ID 416364, 7 pages
http://dx.doi.org/10.4061/2010/416364
Research Article

Mus308 Processes Oxygen and Nitrogen Ethylation DNA Damage in Germ Cells of Drosophila

1Área de Genética, Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (IUOPA), University of Oviedo, 33006 Oviedo, Spain
2Área de Hepatología y Terapia Génica, Centro de Investigación Médica Aplicada (CIMA), University of Navarra, 31008 Pamplona, Spain

Received 13 May 2010; Revised 27 July 2010; Accepted 2 September 2010

Academic Editor: Shigenori Iwai

Copyright © 2010 Nancy Díaz-Valdés et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The D. melanogaster mus308 gene, highly conserved among higher eukaryotes, is implicated in the repair of cross-links and of O-ethylpyrimidine DNA damage, working in a DNA damage tolerance mechanism. However, despite its relevance, its possible role on the processing of different DNA ethylation damages is not clear. To obtain data on mutation frequency and on mutation spectra in mus308 deficient (mus308-) conditions, the ethylating agent diethyl sulfate (DES) was analysed in postmeiotic male germ cells. These data were compared with those corresponding to mus308 efficient conditions. Our results indicate that Mus308 is necessary for the processing of oxygen and N-ethylation damage, for the survival of fertilized eggs depending on the level of induced DNA damage, and for an influence of the DNA damage neighbouring sequence. These results support the role of mus308 in a tolerance mechanism linked to a translesion synthesis pathway and also to the alternative end-joinig system.