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Journal of Nucleic Acids
Volume 2010, Article ID 421803, 3 pages
http://dx.doi.org/10.4061/2010/421803
Research Article

Preparation of DNA Ladder Based on Multiplex PCR Technique

Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Henan 453003, China

Received 14 February 2010; Revised 26 April 2010; Accepted 11 May 2010

Academic Editor: Ashis Basu

Copyright © 2010 Tian-Yun Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

DNA molecular weight standard control, also called DNA marker (ladder), has been widely used in the experiments of molecular biology. In the paper, we report a method by which DNA marker was prepared based on multiple PCR technique. 100–1000 bp DNA fragments were amplified using the primers designed according to the 6631 ~ 7630 position of lambda DNA. Target DNA fragments were amplified using Touchdown PCR combined with hot start PCR, respectively, followed extracted by phenol/chloroform, precipitated with ethanol and mixed thoroughly. The results showed that the 100–1000 bp DNA fragments were successfully obtained in one PCR reaction, the bands of prepared DNA marker were clear, the size was right and could be used as control in the molecular biology experiment. This method could save time and be more inexpensive, rapid, simple when compared with the current DNA Ladder prepared means.