Figure 10: The effect of the position of the THF abasic site lesion within the A4 tract on the insertion and extension by Polη. Templates containing THF abasic site at different positions within the A-tract were synthesized and extended using yeast Polη for 10 to 20 minutes in the presence of 100  𝜇 M dNTPs. In the 10 minutes incubation, the formation of extended product is dependent on the position of the THF lesion. Efficient by-pass of the lesion is observed with the longer incubation time.