Early Steps in the DNA Base Excision Repair Pathway of a Fission Yeast <i>Schizosaccharomyces pombe</i>

Kanamitsu, Kyoichiro; Ikeda, Shogo

doi:https://doi.org/10.4061/2010/450926

Journal of Nucleic Acids

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DNA Damage, Mutagenesis, and DNA Repair

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Review Article | Open Access

Volume 2010 | Article ID 450926 | https://doi.org/10.4061/2010/450926

Early Steps in the DNA Base Excision Repair Pathway of a Fission Yeast Schizosaccharomyces pombe

Kyoichiro Kanamitsu¹and Shogo Ikeda¹

Academic Editor: Ashis Basu

Received21 Jun 2010

Accepted12 Aug 2010

Published16 Sept 2010

Abstract

DNA base excision repair (BER) accounts for maintaining genomic integrity by removing damaged bases that are generated endogenously or induced by genotoxic agents. In this paper, we describe the roles of enzymes functioning in the early steps of BER in fission yeast. Although BER is an evolutionarily conserved process, some unique features of the yeast repair pathway were revealed by genetic and biochemical approaches. AP sites generated by monofunctional DNA glycosylases are incised mainly by AP lyase activity of Nth1p, a sole bifunctional glycosylase in yeast, to leave a blocked end. The major AP endonuclease Apn2p functions predominantly in removing the block. Finally, a DNA polymerase fills the gap, and a DNA ligase seals the nick (Nth1p-dependent or short patch BER). Apn1p backs up Apn2p. In long patch BER, Rad2p endonuclease removes flap DNA containing a lesion after DNA synthesis. A UV-specific endonuclease Uve1p engages in an alternative pathway by nicking DNA on the side of oxidative damage. Nucleotide excision repair and homologous recombination are involved in repair of BER intermediates including the AP site and single-strand break with the block. Other enzymes working in end processing are also discussed.

1. Introduction

DNA molecules in cells always suffer from chemical decay due to exposure to endogenous and environmental agents [1–3]. Cells die when the damage to DNA obstructs replication and transcription. Moreover, base damage causes mutations, which are responsible for cancer, aging, and the hereditary diseases [3–5]. Base excision repair (BER) is a DNA repair pathway directed mainly at nonbulky lesions, such as, alkylated and oxidized bases, and at some types of mismatched bases that are produced during replication or by deamination [3, 5–7]. The BER pathway is usually initiated by DNA glycosylase that removes damaged bases to leave apurinic/apyrimidinic (AP) sites. The AP sites are further processed by an AP endonuclease that cleaves phosphodiether bonds to the AP site to leave a OH and -deoxyribose phosphate (-blocked end). Bifunctional DNA glycosylase associated with AP lyase removes damaged bases and cleaves to the AP site, leaving a --unsaturated aldehyde (-blocked end) and a phosphate. These blocked ends are subsequently converted into OH and phosphate by appropriate enzymes. Finally, a repair DNA polymerase fills the gap, and a DNA ligase seals the nick.

The basic mechanism of BER was first elucidated in Escherichia coli [3]. Subsequent studies showed that the process is conserved in eukaryotes including the budding yeast Saccharomyces cerevisiae and mammals. Fission yeast (Schizosaccharomyces pombe) is an ascomycetous yeast that shares many fundamental cellular properties with higher multicellular eukaryotes [8]. Although S. pombe has been used as a prominent model organism, research of the BER pathway in yeast has started late. Completion of the S. pombe genome project in 2002 provided a list of the yeast BER machineries including DNA glycosylases and AP endonucleases, which are evolutionarily conserved from bacteria to man [9]. Over the past decade, the BER pathway of S. pombe has been fairly well characterized by genetic approaches using many BER-defective mutants constructed by gene targeting. Biochemical properties of BER enzymes were also examined using purified recombinant proteins. In this paper, we describe the roles of the enzymes involved in the early steps of BER in fission yeast with an emphasis on outlining common features as well as differences with other model organisms.

2. DNA Glycosylases in S. pombe

DNA glycosylases identified in E. coli, S. cerevisiae, S. pombe, and the human are classified in Table 1 on the basis of their substrate specificities and structural features [3, 7, 10]. Monofunctional DNA glycosylase simply hydrolyzes the N-glycosidic bond to release damaged bases and leaves the AP site. Nth1 and Ogg1 DNA glycosylases are associated with AP lyase activity, which cleaves to the AP site by elimination, leaving a --unsaturated aldehyde. E. coli Fpg and Nei and human NEILs are other types of bifunctional glycosylases which cleave the AP site by elimination and generate a -phosphate end. S. pombe has five monofunctional DNA glycosylases (Ung1p, Thp1p, Mag1p, Mag2p, and Myh1p) [11]. Unlike other organisms, Nth1p is a sole DNA glycosylase with AP lyase activity in S. pombe cells. Nth1 DNA glycosylase is lyase and does not possess lyase activity [2, 5, 7].

2.1. Uracil DNA Glycosylases Ung1p and Thp1p Function in Avoidance of Spontaneous Mutation

Deamination of cytosine residues in DNA occurs spontaneously in pH- and temperature-dependent reactions, and results in conversion to uracil [1, 3]. Cytosine deamination is estimated to produce 400 uracil residues per mammalian genome per day [12]. Promutagenic U : G mispair causes C : G to T : A transition mutations, if not repaired prior to DNA replication. Uracil also occurs in DNA through incorporation of dUMP instead of dTMP by DNA polymerase during replication. Deamination of 5-methylcytosine in DNA results in the formation of thymine, and hence T : G mispairs. Uracil residues can be removed through the activity of uracil DNA glycosylase (UDG), which consists of four distinct families in mammalian cells: the UNG family (the major activity of UDG in cells), the MUG (mismatch-specific uracil DNA glycosylase)/TDG (thymine DNA glycosylase) family, the SMUG (single strand-specific monofunctional uracil-DNA glycosylase) family, and the MBD4 (methyl-binding domain 4) family [3, 5, 13]. The UDGs, other than MBD4, form a single protein superfamily with a common /-fold structure and must have been evolved from a common ancestor [14].

S. pombe has two UDG genes, ung1 (systematic name SPCC1183.06) and thp1 (SPCC965.05c). The Ung1p protein is highly conserved (51% identity to human UNG) and localizes predominantly to the nucleus [15]. The bacterially expressed protein showed an apparent enzyme activity for uracil-containing DNA, which was inhibited by an inhibitor protein (UGI). Overexpression of ung1 induces a DNA checkpoint-dependent cell cycle delay and causes cell death [15]. Human MUG/TDG was first isolated as an enzyme that excises thymine from T : G mispairs as well as uracil from U : G mispairs [16, 17]. S. pombe Thp1p is a member of the MUG family, and maintains a high level of glycosylase activity towards substrates containing U, 5-fluorouracil, 3,-ethenocytosine, and 5-hydroxyuracil [18]. Unlike human enzyme, Thp1p cannot excise thymine residues from the T : G mispair substrate. Since cytosine methylation is undetectable in fission yeast cells, the potential to process this substrate appears to be correlated with the degree of cytosine methylation in the genome of organisms [19]. Thp1p can also remove deaminated purine bases: xanthine and oxanine from guanine and hypoxanthine from adenine [20]. Both ung1 and thp1 mutants showed a moderate mutator phenotype [21]. Double mutation of the genes additively increased the mutation frequency. Moreover, expression of Ung1p and Thp1p suppressed spontaneous mutagenesis in UDG-deficient cells. These results indicate that both proteins play important roles in the prevention of spontaneous mutagenesis of S. pombe cells.

2.2. Two AlkA Homologs Mag1p and Mag2p Function in Removal of Alkylated Base

Alkylating agents such as methyl methanesulfonate (MMS) generate covalent modifications at nitrogen residues of DNA bases, in particular N3-methyladenine (3-meA) and N7-methylguanine (7-meG), which account for 11% and 83% among the alkylation bases by MMS, respectively [1, 3]. In contrast, 7-meG appears to be a harmless alteration while 3-meA is a cytotoxic lesion that blocks both replication and transcription. S-adenosylmethionine, which is a common cosubstrate involved in methyl group transfer, also takes part in the nonenzymatic methylation of DNA [1]. In a human cell, 3-mA and 7-mG spontaneously generate 600 and 4,000 residues a day, respectively [12].

In E. coli, alkylation products such as 3-meA and 7-meG are mainly removed by two structurally different monofunctional DNA glycosylases, AlkA and TagA [3]. The S. pombe genome encodes two alkA paralogs mag1 (SPAPB24D3.04c) and mag2 (SPBC23G7.11). The amino acid sequences of Mag1p and Mag2p share 44.8% similarity. The S. pombe mag1 gene was cloned by its ability to reverse the MMS-sensitive phenotype of an E. coli alkA/tagA double mutant [22]. The substrate range of Mag1p overproduced in E. coli is limited to the main alkylation products, such as, 3-meA, 3-meG, and 7-meG, whereas no significant activity was found toward deamination products, ethenoadducts, or oxidation products [23]. The efficiency of 3-meA and 3-meG removal was 5–10 times slower for Mag1p than for E. coli AlkA, whereas the two enzymes remove 7-meG at a similar rate. On the other hand, biochemical analysis of Mag2p has not been performed yet because the recombinant protein expressed in E. coli showed no glycosylase activity [23].

Mutant strains and hardly showed MMS sensitivity [23–25]. The BER mutants , , and were sensitive to MMS, while the double mutants , /, and / restored resistance to MMS [23, 25]. This showed that Mag1p is involved in the initial step of MMS-damaged repair both by the Nth1p-dependent short patch BER pathway and by the Rad2p (flap endonuclease)-dependent long patch BER pathway (Figure 1). Moreover, double mutant / was more resistant to MMS than , indicating that Mag2p also functions in the removal of alkylated bases [25]. rad16 encodes an XPF homolog that functions as a DNA repair endonuclease in nucleotide excision repair (NER). The double mutant of rad16 with mag1 or mag2 increased sensitivity to MMS [25]. In addition, the triple mutant exhibited the highest MMS sensitivity. Expression of Mag1p or Mag2p in the triple mutant restored tolerance to MMS. These results showed that BER and NER could independently repair the alkylation DNA damage. Comparison of the substrate specificity and kinetic parameters of Mag1p and Mag2p will be needed to dissect the precise roles of these redundant enzymes in MMS resistance. rhp51 encodes a RecA-like protein that functions in homologous recombination (HR). The double mutant of rhp51 with mag1 decreased the sensitivity to chronic MMS exposure compared with single mutant [24]. In addition, spontaneous intrachromosomal recombination frequencies increased 3-fold in the mag1 mutant [23]. These results show that both Mag1p and HR contribute to repair of the alkylation base. Deletion of mag1 from nth1, rad16, or rad2 decreased the recombination frequency, indicating that AP sites generated by Mag1p removal of spontaneous base lesions are substrates of short patch BER, long patch BER, NER, and HR [23].

Figure 1

A schematic illustration of the BER pathway in S. pombe. In the Nth1p-dependent (or short patch) pathway, repair of the AP site is initiated by incision of Nth1p leaving the -block. Apn2p functions primarily in the removal of the -block, and poorly in the incision of AP sites. Apn1p is a back-up enzyme for -phosphodiesterase activity of Apn2p. Finally, a DNA polymerase (Pol.) fills the gap, and a DNA ligase seals the nick. NER and HR could repair a part of the AP sites and single-strand breaks with blocks. AP endonuclease incises the AP site leaving -dRP end, which will be removed by -dRP lyase activity of Pol4p to feed into short-patch BER. In the long patch pathway, Rad2p incises the flap DNA containing the lesion after DNA synthesis. Uve1p and Apn1p are possibly involved in the NIR pathway. Details are discussed in the text.

2.3. Nth1p Is the Major Contributor for Incision of the AP Site

An 8-oxoguanine (8-oxoG) produced by the oxidation of the guanine residue in G : C could pair not only with cytosine but also with adenine, and the G : C to T : A mutation occurs during the next stage of replication [3, 5, 7]. In a human cell, about 1,000 residues of 8-oxoG are generated per genome in a day [12]. Bifunctional DNA glycosylases Fpg (MutM) in E. coli and OGG1 in budding yeast and mammals can excise 8-oxoG paired with cytosine. Thymine glycol (Tg), which is the oxidation product of thymine, interrupts DNA replication and transcription. In human cells, production of Tg and similar oxidized pyrimidines is estimated to be 500 residues per genome per day [12]. In E. coli endonuclease III (Nth) and endonuclease VIII (Nei) excise Tg and oxidized pyrimidines from DNA and subsequently cleave the strand by AP lyase activity [3]. S. pombe has no homolog of Fpg, OGG1, or Nei [11].

The S. pombe nth1 gene (SPAC30D11.07) was cloned on the basis of homology to E. coli nth [26]. Nth1 family proteins have a helix-hairpin-helix motif in the vicinity of the active lysine residue and an iron-sulfur cluster [4Fe-4S] at the C terminal. In addition, a eukaryote's enzyme has a nuclear localization signal. Indeed, fusion protein of S. pombe Nth1p with green fluorescent protein was predominantly localized in the nucleus [27]. Unlike budding yeast Ntg1p and the enzyme in mammals, S. pombe Nth1p was not observed in mitochondria.

A recombinant S. pombe Nth1p expressed in E. coli shows a broad substrate specificity. Mass spectrometry of released bases from oxidized DNA by treatment of Nth1p revealed that the enzyme efficiently excised five pyrimidine-derived lesions: 5-hydroxycytosine, Tg, 5-hydroxy-6-hydrothymine, 5, 6-dihydroxycytosine, and 5-hydroxyuracil [28]. S. pombe Nth1p could remove 5-formyluracil and 5-hydroxymethyluracil as efficiently as E. coli Nth [29]. Moreover, 8-oxoG in 8-oxoG : G and 8-oxoG : a mispair is also the substrate for S. pombe Nth1p. The expression of S. pombe Nth1p reduced hydrogen peroxide toxicity and the frequency of spontaneous mutations in the E. coli nth/nei double mutant [29]. Although the S. pombe is sensitive to MMS, Nth1p did not remove 3-meA and 7-meG [30]. However, methyl-formamidepyrimidine, a cytotoxic lesion generated from 7-meG by opening the imidazole ring, was excised efficiently by Nth1p. Nicking activity to oligonucleotides containing Tg and the AP site virtually disappeared in the extract from cells, indicating that these lesions are mainly incised by Nth1p in the yeast cell [27].

An strain was tolerant to oxidative damage ( and menadione) and UV irradiation, but exhibited moderate sensitivity to MMS [27, 30, 31]. In addition, the mutant strain exhibited a more than 6-fold increase in the frequency of interchromosomal recombination [30]. Epistasis analysis of the nth1 gene versus rad2, rad16, and rhp55 showed that MMS damage could be repaired through NER and HR other than the BER pathway [30]. Although a mutant of the major AP endonuclease gene apn2 showed hypersensitivity to MMS, double mutation of nth1 and apn2 became tolerant to MMS [31]. This shows that Nth1p functions upstream of Apn2p in the same pathway; that is, the -blocked end generated by Nth1p is converted to OH by phosphodiesterase activity of Apn2p (Figure 1). In S. pombe cells, Nth1p not only removes the oxidized base as DNA glycosylase, but also incises a large portion of the AP site generated by the action of Mag1p and Mag2p DNA glycosylases. Thus, Nth1p plays a central role during early steps of the BER pathway in the fission yeast [27].

2.4. DNA Glycosylase for Mismatch

An 8-oxoG : A mispair in DNA, which is generated by incorporation of an adenine residue opposite to 8-oxoG, leads to a G : C to T : A mutation [3, 5]. The repair of this mismatch is initiated by excision of an adenine residue by adenine-specific mismatch DNA glycosylase (MutY in E. coli). The mutY homolog of S. pombe, namely, myh1 (SPAC26A3.02), has been cloned [32]. Expression of Myh1p in the mutY mutant of E. coli could reduce the spontaneous mutation frequency of the cells. Purified Myh1p recognized A : G and A : 8-oxoG, as well as 2-aminopurine : G and A : 2-aminopurine [32]. In addition, Myh1p probably prevents a C : G to G : C transversion mutation because it can remove the guanine from G : 8-oxoG [33]. The strain displays a 36-times higher frequency of spontaneous mutation than the wild strain [34]. Moreover, showed sensitivity to Myh1p binds to proliferative cell nuclear antigen (PCNA) and PCNA-like heterotrimer Rad9p/Rad1p/Hus1p [35–37]. All subunits of the latter complex are required for proper functioning of the checkpoint and DNA replication under stress [38]. Myh1p may act as an adaptor to recruit checkpoint proteins to the DNA lesion [36].

3. AP Endonucleases in S. pombe

AP sites are generated by removal of damaged bases by monofunctional DNA glycosylase and more frequently by nonenzymatic hydrolysis of the N-glycosidic bond of DNA [1, 3]. In a human cell, about 9,000 residues of the AP sites arise per genome in a day [12]. AP endonuclease hydrolyzes phosphodiether bonds to the AP site to leave OH and -deoxyribose phosphate (dRP). E. coli has two types of AP endonuclease, exonuclease III (Xth), and endonuclease IV (Nfo) [3, 39]. AP endonucleases from various organisms are classified on the basis of structural similarity to Xth and Nfo (Table 2). APE1 in mammals is an Xth-type homolog, which accounts for more than 90% of the AP endonuclease activity in cells [3, 39]. A second Xth-type homolog APE2 was found [40, 41], but no Nfo-type enzymes were present in mammalian cells. On the other hand, yeasts have an Nfo-type homolog Apn1p other than Apn2p with similarity to mammalian APE2. In the budding yeast, Apn1p is the major contributor of AP endonuclease activity [39]. Both Xth and Nfo types of AP endonuclease could also convert blocked ends (e.g., --unsaturated aldehyde, phosphoglycolate, and phosphate termini) to OH. Therefore, -phosphodiesterase and -phosphatase activity of AP endonuclease is essential for the repair of single-strand breaks induced by reactive oxygen species (ROS), ionized radiation, and bleomycin [39, 42].

3.1. Apn2p Predominantly Functions in the Removal of Blocks

The S. pombe apn2 gene (SPBC3D6.10) encodes an AP endonuclease with homology to S. cerevisiae Apn2p and human APE2 [40, 41, 43, 44]. The N-terminal region of the protein has homology with Xth. Notably, Apn2p maintains the entire critical catalytic residue of hAPE1, which is also conserved in several proteins recently predicted to maintain the four-layered / of Xth and the “nonspecific” nuclease DNase I [45, 46]. The C-terminal region of Apn2p protein contains a binding motif similar to PCNA and a sequence homologous to DNA topoisomerase III (TOP3) family [40, 41, 47]. Physical interaction of S. cerevisiae Apn2p with PCNA stimulates the to exonuclease and -phosphodiesterase activities of Apn2p in vitro [47]. However, the PCNA-binding motif and the TOP3-homologous region of S. pombe Apn2p are dispensable for BER of MMS damage in cells [44, 48]. It remains to be elucidated whether Apn2p binds to S. pombe PCNA in vitro and in vivo. Apn2p is predominantly localized in the nucleus [49].

Cells lacking Apn2p exhibit severe hypersensitivity to treatment with MMS and bleomycin analogues [31, 44, 49]. The mutation rate of cells rose remarkably by MMS [44]. Incision activity to oligonucleotides containing an AP site (or tertahydrofuran) was significantly reduced in the extract from cells [44, 49]. These results show that Apn2p is the major contributor of AP endonuclease activity in S. pombe cells. Disruption of nth1 in cells restored tolerance to MMS, indicating that Apn2p functions downstream of Nth1p in the same pathway (Figure 1). Although overexpression of Apn2p in failed to suppress MMS sensitivity, hAPE1 and S. cerevisiae Apn1p reduced the MMS sensitivity of cells to the wild type level [27, 49]. This result suggests that Apn2p cannot begin repair of the AP site in cells in spite of the fact that the enzyme can cleave the AP site in vitro. S. pombe Apn2p predominantly functions in the removal of blocks induced by Nth1p. Trans-complementation of S. cerevisiae Apn1p strongly reflects differences in the major pathway for processing the AP sites between S. cerevisiae and S. pombe cells.

3.2. Other AP Endonucleases of S. pombe

apn1 (SPCC622.17) encodes an Nfo-type AP endonuclease [50]. Although the APN1 mutant of S. cerevisiae is hypersensitive to MMS, the S. pombe apn1 mutant exhibited no sensitivity to MMS and oxidative stress. In addition, the double mutant showed the same MMS sensitivity as the single mutant [30]. However, the double mutant was more sensitive than the single mutant [44]. Moreover, when Apn1p was overexpressed in cells, MMS sensitivity was partially restored [49]. Therefore, Apn1p removes the -blocked end as a back-up function of the major enzyme Apn2p (Figure 1). Since Apn1p was observed in both the nucleus and cytoplasm, it possibly functions in more than just nuclear DNA repair [49]. S. cerevisiae Apn1p has been shown to move into the mitochondria to maintain genomic stability [51].

Uve1p (SPBC19C7.09c), which is primarily a UV-photoproduct specific endonuclease of S. pombe, could recognize non-UV-induced DNA damage (e.g., the AP site and dihydrouracil) and mismatched bases in vitro, and hydrolyze immediately to the damage [52, 53]. The protein has a TIM barrel fold that is very similar to the structure of Nfo [54]. S. cerevisiae has no reported Uve1p homolog. Deletion of the uve1 gene in significantly increased MMS sensitivity [44]. Neurospora crassa Uve1p could complement E. coli with respect to sensitivity to MMS and t-butyl hydroperoxide [55]. The mutation frequency of increased when treated with [56]. This effect was additively elevated by the deletion of apn2, indicating that both Uve1p and Apn2p contribute to the avoidance of -induced mutagenesis, but these enzymes define a distinct oxidative damage repair pathway. E. coli Nfo and S. cerevisiae Apn1p can nick DNA on the side of several oxidized base lesions, generating a OH and a dangling damaged nucleotide, which provide an alternative nucleotide incision repair (NIR) pathway to the classic BER [57, 58]. Therefore, S. pombe Uve1p and Apn1p possibly engage in the NIR pathway by nicking oxidative damage on DNA in an Nth1p-independent manner (Figure 1). In such a case, damaged bases might be removed by a flap endonuclease, Rad2p, after long-patch repair DNA synthesis is primed by the actions of Uve1p and Apn1p [59, 60]. Uve1p works efficiently in mitochondria as well as in the nucleus [61].

3.3. Short- and Long-Patch Repair Subpathways

In the Nth1-dependent repair pathway, -phosphodiesterase activity of AP endonuclease removes -blocked end resulting in a 1-nt gap, which will be refilled via short-patch BER (Figure 1). However, it is not known whether the BER synthesis extends beyond a single nucleotide, with incorporation of two or more nucleotides (long-patch BER). When AP endonuclease incises the AP site, single-strand break with -OH and -dRP ends is generated. The dRP moiety is released by combined action of a DNA polymerase and Rad2p endonuclease (long-patch BER). An X-family DNA polymerase of S. pombe, Pol4p, has been shown to contain an intrinsic -dRP lyase activity like mammalian DNA polymerases and suggesting another AP endonuclease pathway to feed into short-patch BER (Figure 1) [62]. S. cerevisiae Trf4 protein plays a similar role in short-patch BER of the yeast [63]. In mammalian cells, the choice of short-patch or long-patch BER may depend on the state of the terminal moiety and protein-protein interaction of BER components [64]. In vitro analyses of repair synthesis with whole cell extracts or purified enzymes are needed to clarify the relative contribution of short-patch and long-patch BER in S. pombe cells.

4. Enzymes for Removing Blocked Ends in S. pombe Cells

Single-strand breaks with phosphate ends in DNA are induced by chemical attacks of endogenous ROS and environmental oxidants [3]. Fpg/Nei-type DNA glycosylases cleave the AP site by elimination and generate DNA strand breaks with a -phosphate end. In human cells, removal of phosphate is dependent on -DNA phosphatase (hPNK), and not the major AP endonuclease hAPE1 [65]. Unlike bacteria and humans, S. pombe does not possess Fpg/Nei-type DNA glycosylase, and the physiological source of phosphate in the yeast is probably due to the action of tyrosyl-DNA phosphodiesterase I (TDP1). TDP1 catalyzes the hydrolysis of -phosphotyrosyl bonds of the irreversible topoisomerase I (Top1)-DNA covalent complex to generate a -phosphate end [66, 67]. A -DNA phosphatase (Pnk1p; SPAC23C11.04c) has been purified from S. pombe on the basis of its ability to process H₂O₂-damaged DNA to allow DNA synthesis by DNA polymerase [68]. Like human PNK, Pnk1p localizes in nuclei and has both -DNA kinase and -DNA phosphatase activities [69]. The pnk1 mutant showed hypersensitivity to ionizing radiation and camptothecin, an inhibitor Top1, but not to MMS. It remains to be elucidated whether Pnk1p is the major -DNA phosphatase in S. pombe cells.

TDP1 has been implicated in the repair of the irreversible Top1-DNA covalent complex, which can be generated by either exogenous or endogenous factors [67]. Additionally, TDP1 hydrolyses a variety of lesions, including phosphoglycolate [70, 71]. A recessive mutation in the human (TDP1) gene is responsible for the inherited disorder, spinocerebellar ataxia with axonal neuropathy (SCAN1) [72]. A tdp1 (SPCP31B10.05) mutant of S. pombe was completely sensitive to camptothecin [73]. Moreover, the mutant cells progressively accumulate DNA damage and rapidly lose viability in nondividing conditions [73]. Low cellular respiration levels protected the cells, indicating that the production of endogenous ROS is a major cause for the accumulation of DNA lesions in the absence of Tdp1p [73]. Therefore, Tdp1p with Pnk1p processes the same naturally occurring ends, produced from oxidative DNA damage. Rapid and extensive death of the double mutant strain suggested the pivotal role of the HR process in DNA repair in cells [73].

5. BER in a Catalase-Deficient Mutant of S. pombe

The BER mutants of S. pombe are substantially resistant to A catalase-deficient mutant (), in which ROS scavenging activity is extensively reduced, became sensitive to [74, 75]. Deletion of the BER gene (nth1, apn1, apn2, or uve1) from further increased the sensitivity to indicating that catalase activity obscures the functions of BER enzymes in vivo [75]. Double mutants in both ctt1 and BER genes showed extremely high spontaneous mutation rates, especially in the ctt1/nth1 mutant. Vitamin C relieved the mutator phenotype of the ctt1/nth1 mutant [75]. The results provide evidence that BER enzymes as well as catalase and an antioxidant contribute in vivo to avoidance of ROS-induced mutagenesis and cell death.

6. Conclusion

In this paper, we described the early steps of BER in the fission yeast highlighting the key roles of DNA glycosylases, AP endonucleases, and other end-cleaning activities in maintenance of genomic integrity. Although the yeast BER pathway consists of evolutionarily conserved enzymes, the major activity for processing the AP site is different from these of the budding yeast and mammals. AP sites generated by monofunctional glycosylase are mainly repaired via the Nth1p-dependent BER pathway [23, 27, 30, 31]. Unlike other model organisms, the major AP endonuclease of this yeast is assigned to Apn2p, which works primarily in the removal of the block, and poorly in the incision of AP sites [23, 44, 49]. Uve1p engages in an Nth1p-independent pathway by nicking DNA on the side of oxidative damage [56, 75]. Genetic interactions of BER genes with NER and HR genes suggest synergism among the different DNA repair pathways in the protection of alkylation and oxidative damage [23–25, 30]. NER and HR probably repair the intermediates of BER such as, the AP site and single-strand breaks with blocks. BER operates most efficiently when specific protein-protein coordination occurs [76]. A study of physical interaction of the BER proteins will facilitate an understanding of the regulation of BER protein activities and crosstalk between BER and other DNA transaction pathways in yeast cells. Upon nitrogen-starvation, mostly vegetative S. pombe cells promote two rounds of division and enter the state with 1C DNA [77]. The S. pombe state will provide an excellent model to reconsider the roles of BER and other DNA pathways in resting and nondividing physiological conditions [73, 78].

Acknowledgment

This work was supported in part by the Ministry of Education, Culture, Sports, Science and Technology of Japan through a Financial Assistance Program of the Social Cooperation Study (2006-2010).

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Copyright © 2010 Kyoichiro Kanamitsu and Shogo Ikeda. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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