Structural Determinants of Photoreactivity of Triplex Forming Oligonucleotides Conjugated to Psoralens

<table>Binding of TFOs to genomic DNA targets.  2 <svg height="9.6750002" id="M48" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,9.675)">
<g transform="translate(72,-64.26)">
<text transform="matrix(1,0,0,-1,-71.95,66.6)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝜇</tspan>
</text>
</g>
</g>
</svg>M of TFO, either TFO RNA-C2 (lanes 2 and 3) or TFO DNA-C2 (lane 4) were incubated with genomic DNA purified from HT1080 cells and mock irradiated (lane 2) or irradiated with 0.2 J/cm<sup>2</sup> UVA (lanes 3 and 4) and analyzed by single-strand ligation PCR to assay for undamaged (U) and site-specifically damaged (D) DNA.  Lane 1 is a 10 base pair DNA ladder.</table>

Journal of Nucleic Acids

fig3

Figure 3

Figure 3: Structural Determinants of Photoreactivity of Triplex Forming Oligonucleotides Conjugated to Psoralens 