Research Article

Modulation of the Ribonucleotide Reductase-Antimetabolite Drug Interaction in Cancer Cell Lines

Figure 1

Modification of RRM1 expression by stable transfection with RRM1 and shRRM1 expression vectors in cell lines H23, MCF7, and HCT. Wt, wild-type cell lines, R1, clones of cell lines transfected with RRM1; Ct, clones transfected with an out-of-frame RRM1 vector; shR1, clones transfected with a small hair-pin RRM1 vector; shCt, clones transfected with a random control small hair-pin vector. (a) RRM1 protein (red) and mRNA (green) expression in stably transfected clones of H23, MCF7, and HCT8. (b) Western blots of H23, MCF7, and HCT8 clones. (c) Cytotoxicity of MCF7 clones following gemcitabine treatment for 6 days. Each point is the mean of at least three independent experiments. The dashed line indicates the 50% survival fraction.
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