Modulation of the Ribonucleotide Reductase-Antimetabolite Drug Interaction in Cancer Cell Lines
Figure 2
Knock-down of RRM1 and ERCC1 expression in three NSCLC cell lines by RNA interference and impact on gemcitabine efficacy. (a) Western blots showing that RRM1-specific siRNA reduced RRM1 protein expression to undetectable levels, while random control and ERCC1-specific siRNAs did not affect RRM1 expression. Likewise, ERCC1-specific siRNA reduced ERCC1 protein expression to undetectable levels, while random control and RRM1-specific siRNAs did not affect ERCC1 expression. (b) IC50 values of gemcitabine cytotoxicity in cell lines A549, H292, and H460. Wt, wild-type cell lines; si-control, cell lines transfected with nonspecific siRNA; si-ERCC1; cell lines transfected with ERCC1-specific siRNA; si-RRM1, cell lines transfected with RRM1-specific siRNA.
