Journal of Nucleic Acids / 2010 / Article / Fig 1

Research Article

Development of a Novel Fluorescence Assay Based on the Use of the Thrombin-Binding Aptamer for the Detection of -Alkylguanine-DNA Alkyltransferase Activity

Figure 1

Scheme of fluorescence-based hAGT assay. The substrate is the thrombin-binding aptamer modified by an O6-methylguanine (Me) in extended conformation, with a fluorophore and quencher in the opposite ends of the sequence. The refold of the G-quadruplex structure of TBA is dependent on the removal of the methyl adduct by alkyl-guanine-DNA-O6-alkyltransferase (hAGT). This repair moves the quencher and the fluorophore molecules in close proximity and blocks fluorescence.

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