Journal of Nucleic Acids / 2010 / Article / Fig 4

Research Article

Development of a Novel Fluorescence Assay Based on the Use of the Thrombin-Binding Aptamer for the Detection of -Alkylguanine-DNA Alkyltransferase Activity

Figure 4

HPLC analysis of hAGT activity over double- and single-stranded TBA before and after incubation with hAGT. (a) Repair of double-stranded 5-O6-MeG-TBA by hAGT. The peak labelled as cTBA corresponds to the complementary sequence of 5-O6-MeG-TBA. The top right panel shows HPLC chromatogram in absence of hAGT. The gradient was from 10%–40% acetonitrile for 20 min at 6 0 ∘ C . (b) Repair of single- stranded 5-O6-MeG-TBA by hAGT. The inserted panel shows HPLC chromatogram in the absence of hAGT. Gradient used: 10%–40% acetonitrile, 20 min at room temperature.

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