Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana

Figure 4

Selectivity of nucleotide incorporation by ApPol𝜂 opposite undamaged TT (a), CPD (b), 6–4 pp (c), and Dewar (d). ApPolη (12 fmol in lanes 2–7) was incubated with each primer-template in the presence of the four dNTPs (lane 3), the indicated dNTP (lanes 4–7), or the absence of dNTP (lane 2). Lane 1 contained no enzyme. The reactions were incubated at 37^∘C for 5 minutes, and then were subjected to 15% polyacrylamide/7.5 M urea gel electrophoresis. The autoradiograms of the gels are shown.