Role of Topoisomerase II𝛽 in DNA Damage Response following IR and Etoposide

Figure 6

TARDIS analysis of cells treated with etoposide continuously for 24 hours plus and minus MG132 or with MG132 alone. Topoisomerase II-DNA adducts were quantified using an antibody that detects both isoforms. WT MEFs following exposure to 1 𝜇M ± 2 𝜇M MG132 (a), Top2𝛽^−/− MEFs following exposure to 1 𝜇M ± 2 𝜇M MG132 (b), WT MEFs following exposure to 10 𝜇M ± 2 𝜇M MG132 (c), Top2𝛽^−/− MEFs following exposure to 10 𝜇M ± 2 𝜇M MG132 (d), or WT and TOP2𝛽^−/− MEFs following exposure to 2 𝜇M MG132 alone (e). Plots show the mean of the median FITC fluorescence.

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