Error-Prone Translesion DNA Synthesis by Escherichia coli DNA Polymerase IV (DinB) on Templates Containing 1,2-dihydro-2-oxoadenine

Table 1

Nucleotide sequences of oligonucleotides used in this study.

Template 1	3^-CGTCGGGCCCCCTAAGTGATCAAG-5^
Template 2	3^-CGTCGGGCCCCCTA2GTGATCAAG-5^
Template 3	3^-CGTCGGGCCCCCTAGGTGATCAAG-5^
Template 4	3^-CGTCGGGCCCCCTA8GTGATCAAG-5^
Template 5	3^-ACGTCCAGCTCACATCTCCTAG -5^
Template 6	3^-ACGTCCAGCTCACATCFCCTAG -5^
Template 7	3^-ACGTCCAGCTCACATCHCCTAG -5^
Template 8	3^-CGACGGGCCCCCAATGAGAACAAG-5^
Template 9	3^-CGACGGGCCCCCAAXGAGAACAAG-5^
Primer 1	5^-^32P-GCTGCCCGGGGGTT-3^
Primer 2	5^-^32P-TGCAGGTCGACTCTAG-3^
Primer 3	5^-^32P-GCAGCCCGGGGGAT-3^

2, F, H, 8, and X represent 1,2-dihydro-2-oxoadenine (2-oxodA), 5-formyluracil (5-fodU), 5-hydroxymethyluracil (5-hmdU), 7,8-dihydro-8-oxoguanine (8-oxodG), and thymine glycol (dTg), respectively.