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Journal of Nucleic Acids
Volume 2010, Article ID 820356, 8 pages
http://dx.doi.org/10.4061/2010/820356
Research Article

Evaluation of Human Telomeric G-Quadruplexes: The Influence of Overhanging Sequences on Quadruplex Stability and Folding

1Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, P. J. Safarik University, Moyzesova 11, 04154 Kosice, Slovakia
2Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 04001 Košice, Slovakia

Received 11 January 2010; Revised 21 March 2010; Accepted 11 May 2010

Academic Editor: Mateus Webba da Silva

Copyright © 2010 Viktor Viglasky et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To date, various G-quadruplex structures have been reported in human telomeric sequences. Human telomeric repeats can form many topological structures depending on conditions and on base modification; parallel, antiparallel, and hybrid forms. The effect of salts and some specific ligands on conformational switches between different conformers is known, but the influence of protruding sequences has rarely been discussed. In this paper, we analyze different quadruplex-forming oligomers derived from human telomeric sequences which contain - and -protruding nucleotides, not usually associated with the G-quadruplex motif. The study was performed using electrophoresis, CD, and UV spectroscopies. The major findings are (i) protruding nucleotides destabilize the G-quadruplex structure, and (ii) overhanging sequences influence the folding of the quadruplex.