Coincident In Vitro Analysis of DNA-PK-Dependent and -Independent Nonhomologous End Joining
Figure 1
DNA end joining with HeLa, WI-38, and M059K WCEs with and without 5% PEG and/or 10 M wortmannin. (a) Agarose gel image showing typical DNA end-joining reaction results. Dimer and trimer DNA end-joining product yields are calculated as a fraction of the total DNA in the lane and reported graphically as shown in panel B. (b) Graphical depiction of results for triplicate end-joining reactions run with the three cell extracts under each of the conditions indicated. Error bars indicate the standard deviation for triplicate reactions. Significant differences, as determined by the Student t-test, are indicated by an asterisk. The -values for each pairing are given above the brackets.
