Research Article
Pre-Steady-State Kinetic Analysis of Truncated and Full-Length Saccharomyces cerevisiae DNA Polymerase Eta
Table 3
Kinetic parameters of nucleotide incorporation into D-DNA catalyzed by truncated yPol at 23°C.
| | dNTP | kp (s-1) | Kd (M) | kp/Kd(M-1s-1) | Discrimination Facto | Fidelit |
| | Template dA (D-1) | | dTTP | 3.9 ± 0.2 | 15 ± 2 | 2.6 10-1 | | | | dATP | 0.089 ± 0.005 | 80 ± 20 | 1.1 10-3 | 230 | 4.3 10-3 | | dCTP | 0.43 ± 0.06 | 210 ± 60 | 2.0 10-3 | 130 | 7.8 10-3 | | dGTP | 0.15 ± 0.01 | 80 ± 20 | 1.9 10-3 | 140 | 7.2 10-3 |
| | Template dG (D-6) | | dCTP | 15.6 ± 0.3 | 11.2 ± 0.8 | 1.4 | | | | dATP | 0.071 ± 0.002 | 138 ± 9 | 5.1 10-4 | 2700 | 3.7 10-4 | | dGTP | 0.116 ± 0.006 | 80 ± 10 | 1.5 10-3 | 960 | 1.0 10-3 | | dTTP | 0.92 ± 0.07 | 330 ± 40 | 2.8 10-3 | 500 | 2.0 10-3 |
| | Template dT (D-7) | | dATP | 6.9 ± 0.4 | 17 ± 3 | 4.1 10-1 | | | | dCTP | 1.00 ± 0.04 | 210 ± 20 | 4.8 10-3 | 85 | 1.2 10-2 | | dGTP | 0.55 ± 0.01 | 46 ± 3 | 1.2 10-2 | 30 | 2.9 10-2 | | dTTP | 0.62 ± 0.02 | 280 ± 20 | 2.2 10-3 | 180 | 5.4 10-3 |
| | Template dC (D-8) | | dGTP | 6.3 ± 0.1 | 6.8 ± 0.4 | 9.3 10-1 | | | | dATP | 0.087 ± 0.003 | 90 ± 10 | 9.7 10-4 | 960 | 1.0 10-3 | | dCTP | 0.127 ± 0.007 | 200 ± 30 | 6.4 10-4 | 1500 | 6.9 10-4 | | dTTP | 1.39 ± 0.06 | 460 ± 40 | 3.0 10-3 | 310 | 3.3 10-3 |
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