Journal of Nucleic Acids

Research Article

Pre-Steady-State Kinetic Analysis of Truncated and Full-Length Saccharomyces cerevisiae DNA Polymerase Eta

Table 5

Kinetic parameters of nucleotide incorporation into D-1 DNA catalyzed by full-length yPol 𝜂 at 23°C.
dNTPkp (s-1)Kd ( 𝜇 M)kp/Kd ( 𝜇 M − 1   s − 1 )Discrimination Facto r a Fidelit y b
Template dA (D-1)
dTTP4.2 ± 0.540 ± 101.1 × 10-1
dATP 0.0235 ± 0.0003156 ± 71.5 × 10-47001.4 × 10-3
dCTP0.019 ± 0.00170 ± 102.7 × 10-43902.6 × 10-3
dGTP0.043 ± 0.003170 ± 402.5 × 10-44202.4 × 10-3
a C alculated as ( 𝑘 𝑝 / 𝐾 𝑑 ) c o r r e c t / ( 𝑘 𝑝 / 𝐾 𝑑 ) i n c o r r e c t .
b C alculated as ( 𝑘 𝑝 / 𝐾 𝑑 ) i n c o r r e c t / [ ( 𝑘 𝑝 / 𝐾 𝑑 ) c o r r e c t + ( 𝑘 𝑝 / 𝐾 𝑑 ) i n c o r r e c t ] .