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Journal of Nucleic Acids
Volume 2011, Article ID 103872, 6 pages
http://dx.doi.org/10.4061/2011/103872
Research Article

Control of Aptamer Function Using Radiofrequency Magnetic Field

1Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan
2Department of Applied Physics, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan
3Department of Material Science and Technology, Nagaoka University of Technology 1603-1, Kamitomioka, Nagaoka, Niigata 940-2188, Japan
4PRESTO Program, Japan Science and Technology Agency, 5 Sambancho, Chiyodaku, Tokyo 102-0075, Japan

Received 22 May 2011; Accepted 28 June 2011

Academic Editor: Daisuke Miyoshi

Copyright © 2011 Kenichi Taira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Remote control of aptamer function has allowed us to control protein function in space and time. Here, we propose a novel control system for aptamer function by radiofrequency magnetic field- (RFMF-) induced local heating of a gold nanoparticle conjugated with an aptamer. In this study, we used a 31-mer thrombin-binding aptamer (TBA), which can inhibit thrombin activity, as a model aptamer. We evaluated the RFMF control of the inhibitory activity of a gold nanoparticle-conjugated TBA. To evaluate the effect of RFMF on enzymatic activity, we utilized a complementary DNA strand that maintains the broken structure during the activity assay. We observed a decrease in the inhibitory activity of TBA after RFMF irradiation. It indicates that RFMF is capable of controlling the TBA structure. Because RFMF allows noninvasive control of aptamer function, this strategy is expected to be novel way of controlling aptamer drug activity.