Synthetic Pre-miRNA-Based shRNA as Potent RNAi Triggers
Comparison of the RNAi activity between synthetic shRNAs and their corresponding siRNAs. (a) The RNAi activity of siRNAs and shRNAs was analyzed by immunoblotting. (b) The RNAi activity was monitored by a reporter assay system. The luciferase activity ratio for the control siRNA (ctrl) was set as 1. (c), (d) The incorporation of transfected small RNA into the RISC was analyzed by qRT-PCR. Data were normalized against the amount of miR-21 in the RISC. The amount of siRNA incorporated was set as 1. Each graph point in (b), (c), and (d) represents the average (with s.d.) of three independent experiments.