Commercially Supplied Amine-Modified siRNAs May Require Ultrafiltration prior to Conjugation with Amine-Reactive Compounds
Figure 4
SMCC : siRNA ratio can be reduced without loss of yield after ultrafiltration of amine-modified siRNA. Conjugation of amine-modified siRNA with BSA using 40 : 1, 10 : 1, and 2 : 1 SMCC-to-siRNA molar ratios. Conjugates formation was observed for each of the SMCC : siRNA ratios; reducing the ratio from 40 : 1 to 2 : 1 had relatively little effect on conjugate yield. siRNA std : siRNA standard; BSA std : BSA standard.
