Direct Detection of Thrombin Binding to 8-Bromodeoxyguanosine-Modified Aptamer: Effects of Modification on Affinity and Kinetics

Table 1

Binding properties of each TBA.

	𝑘𝑎	𝑘𝑑	𝐾𝐷
	M^−1 s^−1	s^−1	nM
Nonmodified TBA	7.45 ± 0.20; × 10^4	3.38 ± 0.33; × 10^−3	45.4 ± 5.6
8-BrdG substituted TBA	1.58 ± 0.10; × 10^5	3.15 ± 0.46; × 10^−4	1.99 ± 0.29