Research Article

Branched RNA: A New Architecture for RNA Interference

Figure 3

Outline of the synthesis of branched RNA oligonucleotides 1, 2, and 3 with two and four arms with identical sequence. (a) Assembly of the RNA sequence by standard solid-phase methods; (b) removal of protecting groups and release of the RNA molecule from solid support; (c) addition of the symmetrical branching phosphoramidite.
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