Branched RNA: A New Architecture for RNA Interference

Table 1

Mass spectrometry data on modified passenger strand and melting temperatures of siRNA duplexes formed by oligonucleotides 1, 2, and 3 and the linear unmodified control sequences. Buffer conditions: 15 mM HEPES, 1 mM magnesium acetate, 50 mM potassium acetate pH 7.4.

N°	oligonucleotides	MS (expected)	MS (found)	Tm (°C)	Δ𝑇 (°C)
1	(5′TNFα 3′)2-DB	13508	13504	79.8	−3.5
2	(5′TNFα 3′)2-DB-dT	13842	13847	82.5	−1.7
3	(5′TNFα 3′)4-(DB)2-DB-dT	27798	27823	84.3	+0.1
Unmodified	Passenger TNFα	n.d.	n.d.	84.2	0