Synthesis of Specifically Modified Oligonucleotides for Application in Structural and Functional Analysis of RNA

Table 2

Sequences of spin labelled RNAs. The bold U marks the position of 4-thiouridine, and the underlined U the position of 2′-aminouridine.

Name	Sequence	Spin label	Yield (%)
VW1	GGG CUA AAA CAU ACC AGA UUUCGA UCU GGA GAG GUG AAG AAU ACG ACC ACC UAG CUC	TPM-MTS	25
		TMP-IAA	23
VW2	GGG CUA AAA CAU ACC AGA UUUCGA UCU GGA GAG GUG AAG AAU ACG ACC ACC UAG CUC	TPM-MTS	10
		TMP-IAA	63
VW3	GGG CUA AAA CAU ACC AGA UUUCGA UCU GGA GAG GUG AAG AAU ACG ACC ACC UAG CUC	TPM-MTS	20
		TMP-IAA	80
VW4	GGG CUA AAA CAU ACC AGA UUUCGA UCU GGA GAG GUG AAG AAU ACG ACC ACC UAG CUC	TPM-MTS	77
VW5	GGG CUA AAA CAU ACC AGA UUUCGA UCU GGA GAG GUG AAG AAU ACG ACC ACC UAG CUC	TPM-MTS	25
VW6	GGG CUA AAA CAU ACC AGA UUUCGA UCU GGA GAG GUG AAG AAU ACG ACC ACC UAG CUC	TPM-MTS	100
VW7	GGG CUA AAA CAU ACC AGA UUUCGA UCU GGA GAG GUG AAG AAU ACG ACC ACC UAG CUC	TPM-MTS	61
VW8	GGG CUA AAA CAU ACC AGA UUUCGA UCU GGA GAG GUG AAG AAU ACG ACC ACC UAG CUC	TPM-MTS	13
VW9	GGG CUA AAA CAU ACC AGA UUU CGA UCU GGA GAG GUG AAG AAU ACG ACC ACCUAG CUC	TPM-MTS	45
VW10	GGG CUA AAA CAU ACC AGA UUUCGA UCU GGA GAG GUG AAG AAU ACG ACC ACC UAG CUC	TPM-MTS	89
VW11	GGG CUA AAA CAU ACC AGA UUUCGA UCU GGA GAG GUG AAG AAU ACG ACC ACC UAG CUC	TPM-MTS	89
VW12	GGG CUA AAA CAU ACC AGA UUUCGA UCU GGA GAG GUG AAG AAU ACG ACC ACC UAG CUC	TPM-MTS	10
HPAS11	ACC AGA GAA ACA GCC UUA GGA UAU GCU GG	TMC-NHS	81
		TEMPO-NCO	83
HPAS12	CCA GCA GAA GGA CGU CGU AUA UUA CCU GGU	TMC-NHS	80
		TEMPO-NCO	77
HPAS13	ACC AGA GAA ACA ACC UUG GC	TMC-NHS	82
		TEMPO-NCO	97
HPAS14	GGC AAG GUC GUA UAU UAC CUG GU	TMC-NHS	85
		TEMPO-NCO	75
HPAS15	ACC AGA GAA ACA GAC UUG GC	TMC-NHS	72
		TEMPO-NCO	100
HPAS16	GCC AAG UCG UAU AUU ACC UGG U	TMC-NHS	80
		TEMPO-NCO	82