A Potential of microRNAs for High-Content Screening

Table 3

Validation assays in miRNA-based screens.

Effector miRNA	Target genes	Validation assay					Year	Reference
		Expression^a	Reporter^b	qRT-PCR	IS^c	siRNA^d
miR-372 and -373	LATS2	+	+	+	+		2006	[40]
miR-221 and -222	p27^Kip1		+		+		2007	[45]
miR-315	Axin and Notum		+				2007	[110]
miR-34^a	Bcl2 and MYCN				+		2008	[111]
miR-373 and -520c	CD44	+	+				2008	[101]
miR-29^a, b, c	p85α and CDC42		+		+		2009	[38]
miR-192 and -194	Per1, 2 and 3		+				2009	[102]
28 miRNAs	p21Cip/Waf1		+		+		2010	[46]
10 miRNAs	MCL1		+				2010	[99]
miR-1285	p53		+	+	+		2010	[39]
miR-504	p53		+		+		2010	[10]
11 miRNAs	Multiple genes					+	2010	[105]
miR-491	Bcl-xL		+	+	+		2010	[11]
28 miRNAs	p21Cip/Waf1		+		+		2010	[114]

^a mRNA expression profiling.
^b Luciferase and/or GFP reporter assays.
^c Immunostaining.
^d Recapitulation of miRNA-mediated phenotype by selected siRNAs.