Cell-Specific Aptamers as Emerging Therapeutics

Table 2

Aptamers with inhibitory function on target molecules and corresponding selection strategies.

Target	Aptamer	Selection method	Lit.
E-selectin	Thiophosphate-mod. DNA	Two-step selection using recombinant ES-Ig chimera and testing on cells as target	[41]
P-selectin	2′-amino- and 2′-methoxy-mod. RNA	Traditional SELEX using recombinant mouse P-selectin as target	[65]
L-selectin	2′-amino-mod. RNA and DNA	Traditional SELEX using recombinant L-selectin-Ig chimera as target	[37, 38]
IL-17R	DNA	Cell-SELEX using IL-17R presenting NIH3T3 cells as target, unmodified NIH3T3 cells for counter selection	[66]
HER3	RNA	Traditional SELEX using the extracellular part of HER3 as target	[39]
RET	2′-F-mod. RNA	Cell-SELEX using RET^C634Y receptor presenting Pc12/MEN2A cells as target, Pc12 and Pc12/MEN2B cells for counterselection	[50]
NgR	RNA	Traditional SELEX using the extracellular part of NgR-Fc fusion protein as target	[67]
CTLA-4	2′-F-mod. RNA	Traditional SELEX using CTLA-4-Fc fusion protein as target	[68]
gp120	2′-F-mod. RNA	Traditional SELEX using purified recombinant gp120 as target	[69]